Animal Biosafety and Public Health Prevention and Control Technology Innovation Team

I. Research Directions
1.Study on the Pathogenic Mechanisms of Important Zoonoses
Focusing on significant zoonotic diseases such as Brucella, Mycobacterium bovis, and Bacillus anthracis, the team conducts research on the growth metabolism of pathogens and mechanisms of immune evasion. Utilizing the pathogen's transposon random mutation library, in conjunction with high-throughput sequencing technology, the team investigates the functions and regulatory mechanisms of key genes in metabolic pathways such as sugar metabolism and essential metal element metabolism in Brucella and Mycobacterium bovis; the team also elucidates the transmission patterns of Brucella during the resting phase from an ecological perspective.
2.Comprehensive Prevention and Control Technology Research for Important Zoonoses
The team focuses on important zoonotic diseases in cattle and sheep, developing efficient and rapid diagnostic techniques, monoclonal antibody screening technology, and vaccine research and development. Establishing efficient and rapid detection technologies for significant cattle and sheep diseases; establishing reverse genetics and gene recombination technologies for animal viruses; creating recombinant vaccines for Brucella, Mycobacterium bovis, and a third-generation anthrax vaccine.
3.Research on the Prevention and Control Technology of Pet Diseases
The team centers on important pathogens that threaten pet health, such as feline infectious peritonitis virus, feline herpesvirus, and feline calicivirus, to conduct research on pathogenic mechanisms. The focus is on the interaction between the virus and the immune system during the progression of the disease, as well as the development of antibodies and vaccines. By analyzing the structure and function of monoclonal antibodies, the team comprehensively analyzes the composition characteristics of protective antibody responses to the pathogen and uses structural biology to rationally design monoclonal antibodies and vaccines.
II. Representative Achievements
(1) Veterinary Diagnostic Kit and Vaccine
We have developed six distinct and complementary new Brucellosis diagnostic products: Brucella antibody test strips, indirect ELISA antibody detection kit for bovine Brucellosis, competitive ELISA antibody detection kit for animal Brucellosis, IFN-γ sandwitch ELISA detection kit for bovine tuberculosis, Brucella fluorescence polarization antibody detection kit, and indirect ELISA antibody detection kit for sheep Brucellosis. These products have been introduced and applied by international organizations such as EU/OIE/FAO for reference laboratories, and have been awarded the First Prize for Scientific and Technological Achievements by the Chinese Academy of Agricultural Sciences, a Second Prize for Technological Innovation by Da Bei Nong, a Second Prize for Technological Progress by Da Bei Nong, one National Class I New Veterinary Drug Certificate, two National Class II New Veterinary Drug Certificates, and two National Class III New Veterinary Drug Certificates.
(2) Representative Papers and Monographs
1. Han R, Liang L, Qin T, Xiao S, Liang R. Encephalomyocarditis Virus 2A Protein Inhibited Apoptosis by Interaction with Annexin A2 through JNK/c-Jun Pathway. Viruses. 2022 Feb 9;14(2):359.
2. Zhang G, Wang J, Zhao Z, Xin T, Fan X, Shen Q, Raheem A, Lee CR, Jiang H, Ding J. Regulated necrosis, a proinflammatory cell death, potentially counteracts pathogenic infections. Cell Death Dis. 2022 Jul 22;13(7):637.
3. Li S, Chen X, Hao Y, Zhang G, Lyu Y, Wang J, Liu W, Qin T. Characterization of the VP2 and NS1 genes from canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) in Northern China. Front Vet Sci. 2022 Nov 28;9:934849.
4. Zhang G, Li B, Yoo D, Qin T, Zhang X*, Jia Y*, Cui S*. Animal coronaviruses and SARS-CoV-2.Transbound Emerg Dis. 2021 May;68(3):1097-1110.
5. Liu, C., Liu, Y., Li, X., Liang, L*., Cui, S*. Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China[J]. Frontiers in Veterinary Science, 2021, 8.
6. Peng X, Liu Y, Qin Y, Jiang H, Feng Y, Sun J, Niu K, Gao Q, Dong H, Ding J. Comparative Transcriptome Analysis of Artificially Induced Rough-Mutant Brucella Strain RM57 and Its Parent Strain Brucella melitensis M1981. Front Vet Sci. 2020 Jan 10;6:459.
7. Liu Y, Sun J, Peng X, Dong H, Qin Y, Shen Q, Jiang H, Xu G, Feng Y, Sun S, Ding J*, Chen R*. Deletion of the LuxR-type regulator VjbR in Brucella canis affects expression of type IV secretion system and bacterial virulence, and the mutant strain confers protection against Brucella canis challenge in mice. Microb Pathog. 2020 Feb;139:103865.
8. Tang X, Suo J, Liang L, Duan C, Hu D, Gu X, Yu Y, Liu X, Cui S, Suo X. Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system. Vet Res. 2020 Mar 11;51(1):41.
9. Gao X, Guo X, Li M, Jia H, Lin W, Fang L, Jiang Y, Zhu H, Zhang Z, Ding J*, Xin T*. Interleukin 8 and Pentaxin (C-Reactive Protein) as Potential New Biomarkers of Bovine Tuberculosis. J Clin Microbiol. 2019 Sep 24;57(10):e00274-19. doi: 10.1128/JCM.00274-19.
10. Liu Y, Dong H, Peng X, Gao Q, Jiang H, Xu G, Qin Y, Niu J, Sun S, Li P, Ding J*, Chen R*. RNA-seq reveals the critical role of Lon protease in stress response and Brucella virulence. Microb Pathog. 2019 May;130:112-119. doi: 10.1016/j.micpath.2019.01.010.
11. Ding J, Banai M, Yu S, Ting X. Editorial: Veterinary Bacterial Zoonoses. Frontiers in Veterinary Science. 2018 Aug 6;5:162. doi: 10.3389/fvets.2018.00162. 12. Jiang H, Dong H, Peng xw, Feng Y, Zhu L, Niu K, Fan HJ*, Ding JB*. Transcriptome analysis of gene expression profiling of infected macrophages between Brucella suis 1330 and live attenuated vaccine strain S2 displays mechanistic implication for regulation of virulence. Microb Pathog. 2018 June, 119:241-247. doi: 10.1016/j.micpath.
13.Jiang Hui, Feng Yu, Qin Yuming, Zhu Liangquan, Fan Xuezheng, Ding Jiabo. Improvement and application of the microcomplement fixation test for Brucellosis. Chinese Agricultural Sciences. 2022, 55(08).0578-1752.（in Chinese）
14."Veterinary Biologics" (2nd Edition), Xia Yecai, Chen Guanghua, Ding Jiabo. China Agricultural University Press, November 2018.
15."Animal Brucellosis", Ding Jiabo, Dong Hao. China Agricultural University Press, December 2020.
16."Canine Infectious Disease Prevention and Control Technology", Qin Tong. China Agricultural Science and Technology Press, 2019.
17."Pet Coronavirus Prevention and Control Handbook", Qin Tong. China Agricultural Science and Technology Press, 2020.
18."Efficient and Scientific Pigeon Farming and Disease Prevention and Control Technology", Cui Shangjin, Jia Yaxiong, Liang Lin. China Agricultural University Press, 2020.