【Tools link】 iTOL (https://itol.embl.de); Install GTDB-Tk using conda
【Description】
Step 1: Download the strains of Brucella with high-quality complete genome sequencing from Zoonosis, and after screening to remove duplicates, obtain 341 strains. Here are the download links:
Step 2: Rename all the FASTA files to more convenient names for display.
Step 3: The GTDB-Tk software (version 2.4.0) was used in conjunction with its database (version r220) to construct a phylogenetic tree based on the whole genome sequences of these Brucella strains.
Step 4: The tree was subsequently refined using the iTOL online software (https://itol.embl.de) to obtain the final phylogenetic tree.